Questions:1.) The Pyruvate Dehydrogenase Complex is a haloenzyme. What does that mean?2.) List all the levels of protein structure that PDC would have.3.) What is the reaction catalysed by PDC? List all the products.4.) Vitamin B1 is a cofactor in the PDC (ie. It is necessary for enzyme function) explain why one ofthe symptoms of a vitamin B1 deficiency is fatigue.

Pyruvate Dehydrogenase

SOLVED:Regulation of the Pyruvate Dehydrogenase Complex In animal tissues, the rate of conversion of pyruvate to acetyl-CoA is regulated by the ratio of active, phosphorylated to inactive, unphosphorylated PDH complex. Determine what happens to the rate of this reaction when a preparation of rabbit muscle mitochondria containing the PDH complex is treated with (a) pyruvate dehydrogenase kinase, ATP, and NADH (b) pyruvate dehydrogenase phosphatase and Ca^2 (c) malonate.