Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation.

2005年8月26日 · We profiled nucleosome modifications across the yeast genome using chromatin immunoprecipitation coupled with DNA microarrays to produce high-resolution genome-wide maps of histone acetylation and methylation.

Herein, using NMR spectroscopy of nucleosome core particles (NCPs), we evaluate the effects of acetylation of each histone on tail and core dynamics. We show that for histones H2B, H3, and H4, the histone core particle dynamics are little changed, even though the tails have increased amplitude motions.

2014年10月15日 · Recent data suggest that histone modifications also have a direct effect on nucleosomal architecture. Acetylation, methylation, phosphorylation and citrullination of the histone core may...

2024年1月10日 · Acetylation of histones is highly consequential to gene transcription. HATs and HDACs play critical roles in adding and erasing lysine acetylation. Recent cryoEM structures of HAT and HDAC complexes suggest many interesting parallels in how they assemble and function.

Genetic and biochemical investigations of the function the histone tails, and their acetylation, in nucleosome assembly are detailed, followed by an analysis of the importance of histone deacetylation in the maturation of newly replicated chromatin.

2006年7月28日 · Acetylation is one of the most studied epigenetic modifications. It consists of the covalent addition of acetyl groups to the lysines of histone N-terminal tails. This modification not only alters chromatin structure by charge neutralization but may also provide unique binding surfaces for repressors and activators of transcription.

2023年10月4日 · While nucleosomes diffuse ~15% faster in chromatin enriched with H3K27ac, they diffuse ~15% slower in chromatin enriched with RNAP2-Ser5ph. These results argue that high levels of H3K27ac and RNAP2-Ser5ph are not often present together at the same place and time, but rather each marks distinct transcriptionally poised or active sites, respectively.

Recombinant full-length MORF expressed in insect cells and a bacterially produced MYST domain fragment were both able to acetylate free histones in vitro, with a preference for H3 and H4. Furthermore, the insect-derived protein was also competent for nucleosome acetylation, strongly preferring histone H4.

2005年8月26日 · We profiled nucleosome modifications across the yeast genome using chromatin immunoprecipitation coupled with DNA microarrays to produce high-resolution genome-wide maps of histone acetylation and methylation.