根据不同的实验目的使用不同的探针浓度，根据细胞类型和其他的相关因素如细胞或组织的透化等进行调整。 利用培养基或缓冲液将1mM储存液稀释至工作浓度，工作液需现配现用。 常用的工作浓度范围是25-500nM，但对于后续需要进行固定和透化的细胞染色，建议 ...

Mitotracker Red CM-H2Xros是一种还原型的红色荧光染料，本身不发荧光。只需简单孵育细胞，即可被动运输穿过细胞膜，进入细胞后被氧化为发荧光且具线粒体选择性的探针，聚集在活性线粒体上。一旦线粒体被染色后，还能根据后续实验的需求进行固定（醛类固定剂 ...

XT_PGX_MmV1 Immunology kit was used following manufacturer’s instructions. MitoTracker Green FM (1/1500) and MitoTracker Deep Red (1/1000) were added in FACS buffer for 15 min at RT (ThermoFisher ...

ATP, betulinic acid, and staurosporine were purchased from Sigma-Aldrich (St. Louis, MO). MitoTracker Deep Red FM was purchased from Thermo Fisher Scientific (Massachusetts, United States). Antibodies ...

The first-ever U.S. release of a Spring Edition is here! Featuring Grapefruit & Blossom, blending the taste of pink grapefruit with subtle herbal and floral notes.

此前研究已表明，补体体在造血干细胞 / 祖细胞（HSPCs）和骨髓基质细胞中表达，对调节它们的运输、增殖等过程至关重要。然而，补体体缺陷小鼠在造血方面存在诸多问题，比如 C3 基因敲除（C3-KO）小鼠和 C5 基因敲除（C5-KO）小鼠对氧化应激的反应不同，这背后的代谢机制却并不清楚。为了深入探究这些问题，来自华沙医科大学临床前研究与技术中心、路易维尔大学干细胞研究所的 Adrian ...

Red spots on the skin can appear for a variety of reasons, ranging from harmless to potentially serious conditions. They can be caused by simple irritants like dermatitis, infections such as ringworm, ...

Bright red blood in your stool, also known as hematochezia, is a sign of bleeding in the lower digestive tract. It is most likely caused by a condition like hemorrhoids or diverticular disease.

Dylan Strome had a goal and an assist and the Washington Capitals beat the Detroit Red Wings 4-1 on Tuesday night. Recently acquired Mrazek earns first shutout of season as Red Wings blank Knights ...