Figure 1: Confocal imaging of LLC-PK-1 cells in mitosis. To generate the GFP fusion protein, insert the corresponding cDNA into the multiple cloning site of a pEGFP or other appropriate ...

The recent findings of Waldo et al. 1 demonstrate that the fusion of the green fluorescent protein (GFP) to insoluble proteins dramatically reduces its folding ability in prokaryotic cells.

“I’ve never been an applications biologist. I’ve been a discovery kind of biologist.” For the next two decades, GFP remained just an interesting luminescent protein found in some species of marine ...

From growth hormones to cancer drugs, small molecules play a crucial role in our health. Monitoring them is essential to ...

The model was able to predict optimized GFP sequences that had as many as seven different amino acids from the protein ...

A team of scientists in the lab of Nobel Prize winner David Baker has created a method for designing proteins that can bind and sense a range of small molecules, with wide applications for biomedicine ...