Finally, we performed reverse transcription-quantitative polymerase chain reaction (RT–qPCR) to validate the expression of miR-1 and miR-499 in LAA ... qRT-PCR was performed to quantify miRNAs using ...

Note : Support and Resistance level for the month, calculated based on price range of the previous trading month.

The synthesized cDNA was diluted 5 times and then was used as a template for qRT-PCR analysis. The qRT-PCR was performed in a Bio-Rad CFX96 Touch Detection System (BioRad, USA) by using the 2× SYBR ...