参考资料： [1] Liang et al., Transcriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells. Cell (2024) DOI: ...

世界卫生组织数据显示，全球每年约 1930 万癌症新发病例中，1000 万人因癌离世。尽管免疫检查点抑制剂（如 PD-1 抗体）已革新癌症治疗，但仍有大量患者面临 「无响应」 或 「耐药」 难题。其中，自然杀伤细胞（NK ...

《Nature Communications》：CRISPR screen decodes SWI/SNF chromatin remodeling complex assembly 【字体： 大 中 小 】 时间：2025年05月31日 来源：Nature Communications 14.7 ...

卵巢癌 (OC)腹膜转移机制不明导致患者预后极差。南开大学团队通过CRISPR/Cas9全基因组筛选发现NBL1基因在转移灶中显著高表达，证实其通过诱导腹膜上皮细胞间质转化 (EMT)并激活Jak/Stat3通路促进肿瘤转移。该研究颠覆了NBL1作为抑癌基因的传统认知，为靶向治疗提供新策略。

A recent study involving researchers from the University of Basel reveals that slowing down the intracellular transport of ...

CRISPR screens can be performed either with a pooled approach, where all the cells are in large plates, or an arrayed approach, where each well in a multiwell plate contains a different target.

Some CRISPR screens may be missing cancer drug targets. ScienceDaily . Retrieved June 2, 2025 from www.sciencedaily.com / releases / 2024 / 06 / 240615010952.htm ...

CRISPR libraries offer huge advantages for high-throughput screening, including unprecedented editing efficiency, accuracy, and speed. This article walks through the fundamentals of CRISPR screening ...

Some CRISPR screens may be missing cancer drug targets Current CRISPR guides don’t work equally well in cells from people of all ancestries, which could lead to false negative results ...

CRISPR-based screening identifies H2AZ1 as a driver of cellular senescence. (A) Schematic of CRISPR-based loss-of-function ...