ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide ...

designing these probes often requires extensive optimization. Appropriate controls in a qPCR assay ensure reliability and accuracy and help track any potential issues while providing a baseline for ...

Image credit: LGC Biosearch Technologies Design tips ... assays for qPCR gene expression offer the convenience of receiving a customized, dual-labeled BHQplus probe and primer pair in a single ...

Assays specific for a particular target can be designed by using the right primer-probe combination. To help with this task, Exiqon has launched a free online software product to design primers ...

You can supply the specifications, including the probe-to-primer ratio, for gene expression and qPCR assays, and LGC sends you a unique, dual-labeled LNA probe and primer pair in a single tube.