Using ddPCR for CAR T-cell QC Today, scientists mostly rely on quantitative polymerase chain reaction (qPCR) to quantify CAR gene copy numbers, but this technique can only deliver relative results.

Consistent DNA amplification is the cornerstone of high-precision Droplet Digital PCR (ddPCR). High-performance thermal cyclers must deliver uniform temperature control and reproducibility to maintain ...

How ddPCR technology quantifies the empty-full capsid ratio Fluorescence detection in FAM and HEX channels is used in the duplex assay Vericheck ddPCR Empty-Full Capsid Kit.

ddPCR does carry an overall cost that’s double that of RQ-PCR, although the researchers note that more samples per plate can be run by ddPCR (29 vs 25 on a 96-well plate, respectively).

Moreover, GCCL is conducting R&D on various analysis methods, including those for the breast cancer treatment 'Perjeta', using equipment such as 'ELISA' and ddPCR.

All-in-one ddPCR. In other research, scientists at the City University of Hong Kong (CityU) and Shenzhen Futian Research Institute in Guangdong, China, are developing a digital PCR instrument for ...

AUC, analytical ultracentrifugation. *Determined by ddPCR analysis using the QX600 System. Continued analysis distinguished the proportion of capsids carrying the ITR-2 and a contaminating ...

The ddPCR MSI assay can be completed in one day on a ddPCR-based platform to quantify the level of MSI present in colorectal tumors. Using either a blood or FFPE sample, the assay quantitatively ...

Among 54 positive nonquantifiable (PNQ) samples, as determined by Q-RT-PCR in the study, ddPCR detected MRD in 29 while confirming 13 samples as PNQ and proving 12 to be negative.

The new ddPCR method was useful in identifying patients that might be harboring HCC. The research team found that almost 90% of 68 HCC patients were cccDNA-positive compared to 53% of 79 non-HCC ...

In a head-to-head comparison, significantly improved diagnostic accuracy was seen for HPV WGS compared with ddPCR, HPV Ab, and clinical workup (Youden index, 0.99 versus 0.90, 0.83, and 0.82 ...