该研究对用于 CRISPR 基因编辑治疗的 Cas 核酸酶 SaCas9 和 AsCas12a 进行了理性工程化改造，设计出了具有最小化免疫原性的 SaCas9 和 AsCas12a 突变体。

Horizon develops genetically engineered cell models using its suite of DNA editing tools (rAAV, ZFN, CRISPR and Transposon). From this, Horizon offers a range of products and services that enable ...

View Full Profile. Learn about our Editorial Policies. We can target harmful bacteria. We can engineer beneficial bacteria. We can use CRISPR to study microbial interactions. We can use CRISPR to ...

Using a single short RNA, such as a CRISPR guide RNA, to manipulate transcriptional activity at multiple copies of an element should work in theory, says Wysocka. But in practice, “because over ...

Cell 171, 414-426. Initial step of DNA integration by a CRISPR-Cas transposon complex. Bioinformatic analysis has revealed the presence of CRISPR-Cas loci in bacterial Tn7-like transposons, thereby ...

CRISPR is a technology that can be used to edit genes and, as such, will likely change the world. The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell.

Functional genomics can help deconvolute the link between genotype and phenotype in disease, revealing new drug targets and ...