We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green ... a two-step protocol to eliminate ...
The probes are dual-labeled fluorogenic probes that are used in standard real-time PCR protocols ... qPCR fall into two broad categories: intercalating dye–based (predominantly SYBR Green ...
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