Recover the DNA by precipitation with 2.5 volumes of 100% ethanol in the presence of 100 mM NaCl. Vortex vigorously and precipitate at −20 °C for at least 2 h; then collect the DNA by ...
Ethanol-precipitate the DNA before ligation to concentrate the sample and remove salts that can interfere with ligation. The amount of vector obtained is usually sufficient to perform two 260-ng ...
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