A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions. Fusion proteins are powerful tools for the elucidation ...

Figure 1: GFP-fragment reassembly vectors and cloning ... We purify the DNA by electrophoresis through Whatman glass fiber filter paper (GF/C) onto dialysis membrane, but commercial methods ...