Recover the DNA by precipitation with 2.5 volumes of 100% ethanol in the presence of 100 mM NaCl. Vortex vigorously and precipitate at −20 °C for at least 2 h; then collect the DNA by ...
Avoid fusions to the GFP fragments at the site of protein-protein interaction ... and recover the DNA by precipitation with ethanol. Analyze the size and quality of the products by electrophoresis ...
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