Nature1 年
Detecting protein-protein interactions with GFP-fragment reassembly
Avoid fusions to the GFP fragments at the site of protein-protein interaction ... and recover the DNA by precipitation with ethanol. Analyze the size and quality of the products by electrophoresis ...
Nature3 个月
Profiling DNA methylation patterns using genomic tiling microarrays
Recover the DNA by precipitation with 2.5 volumes of 100% ethanol in the presence of 100 mM NaCl. Vortex vigorously and precipitate at −20 °C for at least 2 h; then collect the DNA by ...