1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

While researchers have long relied on electrophoretic mobility-shift assays (called gel shifts) to identify which transcription factors bind to DNA samples, the method can be time-consuming and ...

Scientists who study protein-DNA interactions frequently rely upon gel shift assays or electrophoretic mobility shift assays (EMSAs), which are based on relative differences in electrophoretic ...