The standard Cas9 protein cuts the DNA at the target. When the cut is repaired, mutations are introduced that usually disable a gene. This is by far the most common use of CRISPR. It’s called ...

Cas9 activity linearized the DNA circles, and the team sequenced the linear fragments, revealing the sequences where the enzyme cut. The team showed that DNA gyrase ... an additional mechanism by ...

Early reports have demonstrated that CRISPR effectively excises integrated human immunodeficiency virus type 1 (HIV) from cells. 1,2 For Michele Lai from the University of Pisa, these studies raised ...

1 CRISPR-Cas systems utilize RNA guides to direct Cas proteins to specific DNA sequences, where the Cas protein cleaves the DNA. This ability to precisely target and cut DNA is the foundation for ...