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In this form of pulsed-field gel electrophoresis ... Connect the gel apparatus to an appropriate power supply set to deliver 4-s pulses at a constant current of 170–180 mA for 30 min.
Contour-clamped homogeneous electric field (CHEF) electrophoresis is a technique of pulsed-field gel electrophoresis that enables the resolution of large fragments of DNA that cannot be resolved ...
Large DNA Fragment Analysis: Constant- vs Pulsed-Field Electrophoresis Technologies. App Note / Case Study . Last Updated: October 11, 2023 (+ more) ...
Please use one of the following formats to cite this article in your essay, paper or report: APA. Heidolph North America. (2021, May 28). Pulsed-Field Gel Electrophoresis (PFGE) for the Separation ...
When the technique of pulsed-field gel electrophoresis (PFGE) was first described by David C. Schwartz and Charles R. Cantor almost a decade ago (Cell, 37:67-75, 1984), many molecular biologists ...
For a 30-year-old experimental method, gel electrophoresis remains popular. Originally devised as a method to extract nucleic acids from solution, it has evolved into a method for the analysis of ...
Capillary gel electrophoresis (CGE) Like CZE, CGE requires constant field strength and is dependent on the pH of the buffer solution as the particles move through the gel and separate out based on ...
5B10.XX Gel Electrophoresis. Difficulty Moderate: Setup Time Short: Visibility Excellent: Appeal ★★★ Education ★★★ Concepts. Electric fields, diffusion. Overview. When dyes are in a gel in a strong ...
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