This comprehensive guide helps scientists keep up with the latest and greatest protein blotting approaches and technologies.

The Southern blot is the most common way to analyse restriction ... The first way is to cut 3 asymmetric notches on the edges of the filter before it is placed on the plate. Once the membrane has been ...

The main steps in a western blot protocol are as follows. Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins ...

Following separation, the proteins are transferred from the gel onto a blotting membrane. Although this step is what gives the technique the name "western blotting," the term is typically used to ...

These filters can be obtained from Omega Optical ... binding of the primary antibody and the auto-fluorescence of the blotting membrane. Several protein-antibody combinations have shown ...