The Southern blot is the most common way to analyse restriction endonuclease-digested ... The first way is to cut 3 asymmetric notches on the edges of the filter before it is placed on the plate. Once ...

These filters can be obtained from Omega Optical ... binding of the primary antibody and the auto-fluorescence of the blotting membrane. Several protein-antibody combinations have shown ...

It is analogous to Southern blotting, which is used to analyze DNA. This protocol describes the transfer of RNA from agarose gels to nylon membranes and the fixation of the RNA to the membrane 1,2,3.

The main steps in a western blot protocol are as follows. Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins ...